Bacillus spp. were incorporated into the diet by centrifugation of the
inoculumat 4000 ×ǥ for 10min at 4 °C, then 400mL of the inoculum(at
a concentration of 107 CFU·mL−1) was sprayed on 400 g of feed, transferred
to an ovenwith air circulation (Marconi, Piracicaba, SP) for drying
at 30 °C for 24 h, reaching the concentration of 1.15 × 104 CFU·g−1.
After drying, 1% cod liver oil (LabSynth® Diadema, SP) was added to
both diets for pellet protection.
Bacillus spp. were incorporated into the diet by centrifugation of theinoculumat 4000 ×ǥ for 10min at 4 °C, then 400mL of the inoculum(ata concentration of 107 CFU·mL−1) was sprayed on 400 g of feed, transferredto an ovenwith air circulation (Marconi, Piracicaba, SP) for dryingat 30 °C for 24 h, reaching the concentration of 1.15 × 104 CFU·g−1.After drying, 1% cod liver oil (LabSynth® Diadema, SP) was added toboth diets for pellet protection.
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