It has also been reported that spectrophotometric methods tend
to overestimate carotenoid content when compared to HPLC due to
other compounds also detected, for example, carotenoid degradation
products (Kimura, Cobori, Rodriguez-Amaya, & Nestel, 2007)
and chlorophyll degradation products such as chlorophyllides
which are also absorbing at similar wavelengths (Almela,
Férnandez-Lopéz, & Roca, 2000) despite the fact that their
increased polarity due to phytol cleavage would result only in
small extractability in hexane (Chiba et al., 1967).