The cells from stock cultures were transferred to 15 ml of
growth medium in a 20ml vial, which was then incubated at
38 ◦C for 12 h on a shaking incubator (KMC-8480SF, Vision
Scientific Co., Daejon, Korea) at 200 rpm. A 0.6 ml of this
culture was transferred again to a 20ml vial containing fresh
growth medium. After three or four consecutive transfers,
a 2 ml of final culture was transferred to 40 ml of growth
medium in a 50ml vial, which was then incubated at 38 ◦C
and 200 rpm for 6 h before inoculation to the fermentor at 4%
(v/v).
The cells from stock cultures were transferred to 15 ml ofgrowth medium in a 20ml vial, which was then incubated at38 ◦C for 12 h on a shaking incubator (KMC-8480SF, VisionScientific Co., Daejon, Korea) at 200 rpm. A 0.6 ml of thisculture was transferred again to a 20ml vial containing freshgrowth medium. After three or four consecutive transfers,a 2 ml of final culture was transferred to 40 ml of growthmedium in a 50ml vial, which was then incubated at 38 ◦Cand 200 rpm for 6 h before inoculation to the fermentor at 4%(v/v).
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