(Figure 1). It was followed by 0.1
mg/l IAA producing 70% shoot initiation, an average of
3.5 shoots per explant, 4.6 cm long shoots, 8.8 leaves
and 5.0 nodes per shoot. Whereas, the least response
was found in control in all the parameters studied. This
might be due to the fact that IAA is not usually considered
to be a shoot proliferation growth regulator rather than
root promoting regulator, as reported by Lazar and
Cachita (1983). Our results gets support from a previous
work done by Rout et al. (1997) who stated that the lower
the indole acetic acid concentration, the higher would be
the shoot bud regeneration in chrysanthemum. These results
are in agreement with our previous findings
(Waseem et al., 2008) who also reported that a low concentration
of IAA (0.3 mg/l) excelled all the other concentrations
by producing the best results for shoot
proliferation.
Effect