In this study, the effects of agitation and aeration on mycelial growth and exo-polysaccharide production were examined
in batch cultures of Tricholoma matsutake. Agitation was varied from 100 to 300 rpm and aeration was varied from 0.5 to
1.5 vvm. Mycelial growth was 21.87 g/l at 150 rpm, and exo-polysaccharide production was 8.79 g/l at 1.5 vvm. When we
analyzed the polysaccharide extractions from the cultured mycelium and the culture broth of T. matsutake, 1.4 g of crude
polysaccharide was found per 100 g of dried weight in the cultured mycelium, and 1.47 g/l of polysaccharides was found in the
culture broth. In addition, the amounts of β-Glucan in the soluble polysaccharide fractions of the cultured mycelium and
culture broth were 75.4% and 83.6%, respectively. The cultured mycelium and the culture broth contained a higher amount of
β-Glucan than that of the fruiting body.
In this study, the effects of agitation and aeration on mycelial growth and exo-polysaccharide production were examinedin batch cultures of Tricholoma matsutake. Agitation was varied from 100 to 300 rpm and aeration was varied from 0.5 to1.5 vvm. Mycelial growth was 21.87 g/l at 150 rpm, and exo-polysaccharide production was 8.79 g/l at 1.5 vvm. When weanalyzed the polysaccharide extractions from the cultured mycelium and the culture broth of T. matsutake, 1.4 g of crudepolysaccharide was found per 100 g of dried weight in the cultured mycelium, and 1.47 g/l of polysaccharides was found in theculture broth. In addition, the amounts of β-Glucan in the soluble polysaccharide fractions of the cultured mycelium andculture broth were 75.4% and 83.6%, respectively. The cultured mycelium and the culture broth contained a higher amount ofβ-Glucan than that of the fruiting body.
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