Fig. 5. (A) Cumulative methane and (B) gas production within 14 days of anaerobic
incubation depending upon inoculation with T. viride and aerobic pre-incubation
time. c 3, c 10, c 20; controls with a three-, ten, and twenty-day pre-treatment. Tv 3,
Tv 10, Tv 20; T. viride with a three-, ten, and twenty-day pre-treatment. Data points
are means (±SD), n = 3. (C) Interaction plot of a multivariate ANOVA showing the
impact of pre-treatment with T. viride and varied pre-incubation periods on
methane yield after 14 days of anaerobic treatment. Significant differences
(p < 0.05) are indicated by different characters. Bars indicate 95% confidence
intervals.