This research investigated growth of algae from different natural sources to compare the growth rate of algae and studied the possibility to produce natural oils from algae. the study use water from pond in front of Faculty of Engineering Kasetsart University,pond opposite Faculty of Engineering Kasetsart University and pond in front of store in Faculty of Engineering Kasetsart University. we use Tris-Acetate-Phosphate medium and Tris-Acetate-Phosphate agar medium as source of nutrient