where V- volume of alcoholic NaOH solution (L); M- molarity
of NaOH solution; MFFA- molecular weight of FF A (g/mol); wweight
of the oil sample (g). In the fmal step, the FF A was
converted to FAME by acid catalyzed esterification reaction
with methanol at 60°C and atmospheric pressure. Typically, 50
g FF A was put into a three necked 500 mL round bottom flask
equipped with a reflux condenser and rubber septum. The flask
was immersed in an oil bath with a temperature controller and
magnetic stirrer preheated to required temperature. Required
amount of H2S04 was mixed with methanol and transferred
into the reaction medium with syringe. Samples were
withdrawn in every 10 min and FF A content was analyzed.
After 50 min when the FF A content reduced to