Sera were collected at random from four different breeder flocks
(Table 2) of Tamilnadu which were vaccinated subcutaneously
against IBH/HPS with a commercial oil adjuvanted inactivated
vaccine (FAdV-4) at 2nd week of age (dose 0.3 ml) and booster vaccination
was done at 16th weeks age (dose 0.5 ml). Twenty sera
samples were collected randomly from 16th weeks old, 24th weeks
old and 32 weeks old flocks but only 19 sera samples were collected
from 14th weeks old flock. Sera samples were screened by ELISA,
Dot ELISA and results were compared with AGID test results.