At the end of the third week, all groups were treated for 10 minutes
as follows: group 1, immersed in 3 mL of Triphala (60 mg/mL in 10%
DMSO); group 2, immersed in 3 mL of GTP (60 mg/mL in 10%
DMSO); group 3, immersed in 3 mL of MTAD; group 4, immersed in 3
mL of 5% NaOCl; and group 5: immersed in 3 mL sterile saline. Then,
the biofilm on the root canal portion was scraped and inoculated on Mueller-
Hinton agar plates and incubated for 24 hours at 37C for qualitative
analysis where n = 5 for each group. The quantitative analysis was performed
by vortexing the tooth samples with sterile saline for a few minutes
followed by serial dilution method for all the groups where n = 10 for
each group. The same procedure was repeated for all groups once again
at the end of the sixth week to analyze qualitatively and quantitatively