were collected tissue samples were taken from the lungs of infected pigs displaying clinical symptoms
immediately frozen at -80 ˚C.
TTSuV1-positive pigs, obtained from a PRRSV-eradicating farm
antigen negative for swine influenza virus, pseudorabies virus,classical swine fever virus, PCV2 and porcine parvovirus byPCR (or RT-PCR) method.
all samples were washed three times with DEPC water
all samples were stored at 20 8C until analyzed.