This study applied bioaugmentation technology for in situ remediation
of the high concentration BTEX-contaminated groundwater
with approximately 100 mg l−1 in total concentration for the first
time. For evaluating the potential of bioaugmentation in remediation
of high concentration BTEX-contaminated groundwater,
five aspects studies were conducted, (1) efficiency of immobilized
Mycobacterium sp. CHXY119 beads for biodegrading high concentration
BTEX-contaminated groundwater contained in shaking
flasks; (2) performance improvement in biodegrading high concentration
BTEX-contaminated groundwater by the Mycobacterium
sp. CHXY119 and Pseudomonas sp. YATO411 immobilized bead in
shaking flask; (3) relationship between biodegradation efficiency
of BTEX and rate of oxygen consumed by mixed cultures in immobilized
beads in batch experiment; (4) performance of the mixed
cultures immobilized bead for bioremediation of high concentration
BTEX-contaminated groundwater in a PRB using ORCs as slow
release source for oxygen supply; (5) toxicity decrease evaluation of
the BTEX-contaminated groundwater in the PRB using luminescent
bacteria toxicity test.