A simple and sensitive QuEChERS method for the determination of imidacloprid residues in mulberry leaf was developed using high performance liquid chromatography (HPLC). This method was then evaluated for the residual level and dissipation rate of imidacloprid in mulberry leaves. A maximum initial deposits of imidacloprid on mulberry leaves was found to be 3.047 μg g -1 and 6.490 μg g-1 following two application rates of imidacloprid 17.8 SL at 0.6 and 1.2 mL L-1. The calculated half-life of imidacloprid in mulberry leaves was at recommended dose 3.81 d with a dissipation rate of 76% over 7 d; whereas, in double, the dose was 4.93 days with a dissipation rate of 84% over 10 d. Copyright