In B. forficata, two studies exist. Shoots have
been shown to originate from cotyledonary node
epidermal and subepidermal tissue through a process
of indirect organogenesis and amitosis (nuclear
fragmentation) (Appezzato-da-Glória and Machado
2004). In that study, half-strength MS (½ MS)
basal medium to which 3% (w/v) sucrose and
4 mg·dm-3 BA were added allowed callus to form
from 1 cm long hypocotyls after 30 days when
cultured under 30 μmol·m
-2
·s
-1
PPFD with a 16-h