Callus development with three parameters were studied i.e. callus
initiation (in days), callus colour and callus texture (Table 1). Callus formation
was observed in all explants except roots under all experimental conditions.
In IAA explants responded by initiating callus in all IAA concentrations
except 1mg/L. In 1mg/L only petiole and leaf developed callus whereas internode
and node did not respond. Maximum explants initiated callus formation in 20 day.
The callus initiation process decreased when time period was increased or
decreased from 20 days. Minimum days recorded were 17 days when leaf blade
developed callus whereas maximum days consumed for callus initiation were
taken by node were 24. In all explants callus colour was green or shades of
green. Mostly it was green (14 readings) followed by yellow green (4 readings)
light green (3 readings) and bright green (1 readings only). Texture of the callus
was explant oriented. Under all IAA concentrations, explants petiole and leaf,
produced granular callus whereas internodes and nodes produced compact
callus.