A wheat-based dosa made of rice and wheat flour was prepared
and used as a food system in this study as follows. A
29.7 g of rice flour was washed and boiled with 60 mL distilled
water for 10 min in a 200 mL beaker. Forty five grams of
wheat flour and 9.9 g skim milk powder (separated from cow’s
milk, spray dried, V.I.S. Foodtech, Malaysia) were separately autoclaved
and then mixed in the fermentation tank. A portion
of 84.6 mL sterile distilled water was added to the mixture in
the fermentation tank. Finally, the fermentation tank containing
the whole mixture was autoclaved again to provide a
sterilized medium for the growth of desired lactic acid bacterium.Therefore,
L. plantarum MNZ (4.36 × 108 CFU/mL) was then
transferred into the bioreactor (Sartorius BBI Systems, Göttingen,
Germany) under sterilized conditions. The bacterium was
then allowed to ferment the dosa ingredients at an ambient
A wheat-based dosa made of rice and wheat flour was preparedand used as a food system in this study as follows. A29.7 g of rice flour was washed and boiled with 60 mL distilledwater for 10 min in a 200 mL beaker. Forty five grams ofwheat flour and 9.9 g skim milk powder (separated from cow’smilk, spray dried, V.I.S. Foodtech, Malaysia) were separately autoclavedand then mixed in the fermentation tank. A portionof 84.6 mL sterile distilled water was added to the mixture inthe fermentation tank. Finally, the fermentation tank containingthe whole mixture was autoclaved again to provide asterilized medium for the growth of desired lactic acid bacterium.Therefore,L. plantarum MNZ (4.36 × 108 CFU/mL) was thentransferred into the bioreactor (Sartorius BBI Systems, Göttingen,Germany) under sterilized conditions. The bacterium wasthen allowed to ferment the dosa ingredients at an ambient
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