To detect the phosphate solubilizing bacteria, strains were
streaked onto Pikovskaya's agar medium, which contains (per
liter): 0.5 g of yeast extract, 10 g of dextrose, 5 g of Ca3(PO4)2, 0.5 g
of (NH4)2SO4, 0.2 g of KCl, 0.1 g of MgSO4 ×7H2O, 0.0001 g of
MnSO4·H2O, 0.0001 g of FeSO4×7H2O and 15 g of agar. After 3 d of
incubation at 28°C