3.3. Bacterial isolation
Only 10 isolates of M. hyopneumoniae were recovered
out of the 160 lungs cultured. Of those, 9 mixed cultures of
both M. hyopneumoniae and M. hyorhinis were observed. M.
hyopneumoniae was recovered in pure culture in only one
lung. No M. flocculare was recovered whereas two pure
cultures of M. hyorhinis were isolated from two different
lungs. All positive broth cultures (n = 12) were tested by
multiplex PCR for the identification of M. hyopneumoniae,
M. hyorhinis, and M. flocculare (Table 1). The overall
isolation rate for both slaughterhouses was 6.2% for M.
hyopneumoniae and 6.9% for M. hyorhinis. Interestingly, the
recovered isolates were all from slaughterhouse #2
(n = 12/50) indicating an isolation rate for this unit of
20% for M. hyopneumoniae (n = 10/50) and of 22% for M.
hyorhinis (n = 11/50).