The cultures grown in different broths were centrifuged at
1000 g for 10 min at 4 C,washing twice and re-suspending in PBS.
Volume (0.1 ml) of each suspension containing approximately
107 cells/ml, as determined by a haemocytometer slide at a
magnification of 400 on a Leica semi-motorized research microscope
(Model DM3000; Germany),