Statistical Analysis
The extraction and recovery experiments were performed in triplicate.
Statistical analysis was performed using mean separation by Duncan's
Multiple Range Test at a 5% confidence level.
RESULTS
Separation of Organic Phosphorus Compounds
To achieve separation between ATP, ADP and AMP using the same
mobile phase composition, the amount of acetonitrile was decreased from 17
to 7%, which increased the retention time of ATP at a flow rate of 1 mL min -1 ,
but also broadened the peak somewhat. This was overcome by increasing