Fruit trees are considered to be recalcitrant material for genetic transformation studies and the main impediment for genetic trans- formation is the regeneration of transformed plantlets. Choice of explants having competence for transformation and regeneration is a crucial factor. Hence, efficient tissue culture techniques become the base for genetic transformation studies (Giri et al., 2004). Genetic transformation studies aimed at improving morphological and growth characteristics of the plants are however hindered by low transformation efficiencies and genotype dependence of pro- tocols. Guava regeneration studies are an essential complement of transformation studies.