The effects of the cells loading, immobilized cell efficiency, cell
viability and reusability on the reaction yield on the entrapped
S. cerevisiae were studied following the kinetic behavior of con-
secutive cycles of fermentation in batch reactions systems for
free and immobilized yeast. Important parameters such as ethanol
yield, productivity, sugar consumption and release biomass were
reported for each reaction cycle. High ethanol yield and cells via-
bility is observed during consecutive five fermentation cycles. The
results suggest that the simple procedure described could be used
to prepare reusable isolated biocatalysts for bioethanol production.
We believe that, monolithic macroporous hydrogel-yeast catalyst
becomes a versatile, biocompatible and reusable heterogeneous
catalyst, as well as enable simplified downstream processing, able
to isolate valuable strains; achieving a more eco-friendly and eco-
nomically convenient process, without radical modifications of
equipment and devices involve in the classical operation mode