Appressoria were produced using a slide
culture technique, in which 10 mm2 squares of
PDA were placed in an empty Petri dish. The
edge of the agar was inoculated with spores
taken from a sporulating culture and a sterile
cover slip was placed over the inoculated agar
(Johnston and Jones, 1997). After 3-7 days, the
shape and size of the appressoria formed across
the underside of the cover slip were studied.