Viroids were extensively studied in the past few years, and new
insights not only into their propagation, in vivo replication, processing,
trafficking, and pathogenesis, but also into their tertiary structure and
interactions with cellular proteins or small RNA were reported.However,
several questions regarding the mechanisms by which these viroids
enter and leave the cell, the nucleus or the chloroplast and escape the
host degradation system, remain unclear. Indeed the prevention of
viroid infections in plants is so far based on barely biological means
and no chemicals are available to control or prevent plant diseases
caused by viroids. The current approaches used to combat viroids are
the elimination of source inoculum, prevention of secondary spread,
cross-protection, and the use of crops bearing resistance traits [9].
Nevertheless, one way of preventing Avsunviroidae infection could be
to break their rolling circle replication by inhibiting their hammerhead
self-cleavage, perturbing thus the equilibrium between linear, circular,
and polymeric viroids in infected cells, and allowing the defense mechanism of the cell, in particular the nucleases, to recognize and
degrade them. This idea has been put forward by Murray and Arnold
who showed that tetracycline is a potent inhibitor of HHR
Viroids were extensively studied in the past few years, and new
insights not only into their propagation, in vivo replication, processing,
trafficking, and pathogenesis, but also into their tertiary structure and
interactions with cellular proteins or small RNA were reported.However,
several questions regarding the mechanisms by which these viroids
enter and leave the cell, the nucleus or the chloroplast and escape the
host degradation system, remain unclear. Indeed the prevention of
viroid infections in plants is so far based on barely biological means
and no chemicals are available to control or prevent plant diseases
caused by viroids. The current approaches used to combat viroids are
the elimination of source inoculum, prevention of secondary spread,
cross-protection, and the use of crops bearing resistance traits [9].
Nevertheless, one way of preventing Avsunviroidae infection could be
to break their rolling circle replication by inhibiting their hammerhead
self-cleavage, perturbing thus the equilibrium between linear, circular,
and polymeric viroids in infected cells, and allowing the defense mechanism of the cell, in particular the nucleases, to recognize and
degrade them. This idea has been put forward by Murray and Arnold
who showed that tetracycline is a potent inhibitor of HHR
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