It is a problem solving activity that requires experiencing the spatial layout of a building, discovering and learning spatial information, and maintaining spatial orientation during navigation. Lifelong learning is a must in our society with changing spatial environments and user needs. While developing the spatial organization of an environment during the initial phase of the design process, interior architects need to determine the nature of the way finding problems that future users will encounter during navigation (Passini, 1996). With the emergence of three-dimensional (3D) virtual environments (VEs), interior architects are able to obtain an immersed view of the proposed building, assess and improve their designs by learning from the navigation behaviors of the users. This study focuses on the issue of spatial learning during virtual navigation in a multi-level desktop VE designed in SL and investigates the usability of SL as a tool for spatial learning in interior architecture.