C. Preparation of Bacterial Consortia
To prepare successful microbial consortium, bacterial
cultures must be compatible with each other in order to
concomitantly produce all these enzymes required for the
degradation of kitchen wastes. 15 different consortia were
prepared and incubated overnight at 37 oC in 120 rpm. The
compatibility of the bacterial strains within the consortia was
checked by gram staining. Microbial consortium was
prepared by inoculating 5 over night grown bacterial strains
in 20ml of nutrient.