The aim of this study was to ascertain whether food-isolated yeasts possessed biocontrol activity against the pathogenic fun- gus B. cinerea. Thus, the biocontrol activity of different yeast species and strains isolated from fruit and olive brine was evaluated in vitro conditions and on grapes. Furthermore, the effects of iron (Fe3þ) availability, the production of extracellular lytic enzymes, VOCs and biofilm formation, as well as the ability to colonize the fruit wounds were investigated for each species.