In this study, we focused on hydrophilic antioxidants potentially
present in the polar-soluble crude extracts from defatted sesame
seeds. Therefore, we attempted to separate the polar-soluble
crude extracts into different saccharide fractions. Extracts were
analyzed by HPLC gel filtration column chromatography (YMCPack
Diol 60) and the eluted saccharide fractions were identified
using a refractive index detector (RID). Chromatograms of white
and gold sesame seed extracts are illustrated in Fig. 3A and B. Both
extracts were separated into two main saccharide fractions. The
eluted peaks were identified by comparison with standard oligosaccharides
separated under the same HPLC conditions (Fig. 3C).
The first fraction, designated as F1 was obtained with 17.1% and
17.5% yield from white and gold sesame seed polar-soluble crude
extracts, respectively. This fraction corresponded to relatively high
molecular weight saccharides, such as polysaccharides or other