The filtrate was analyzed spectrophotometrically at kmax 250 nm. Concentration obtained
was multiplied by the total volume of the dispersion produced,
considering the dilution factor. This represented the concentration
of free drug (Cf, namely that not entrapped in cubosomes). This
was then subtracted from the total drug concentration (Ct) in the
formulation to give the amount of drug that was successfully entrapped
inside the cubosomes. Each experiment was repeated
three times.