The survival of V. parahaemolyticus phages was tested in marine
water of the aquaculture system Corte das Freiras, in three different
dates, between May and July 2013. In each date, 50 mL of water were
filtered through 0.45 μm and then by 0.22 μm pore-size membranes
(Poretics, USA) which was followed by the addition of phage suspensions
of about 107 PFU mL−1. The samples were then incubated at
25 °C without shaking, in the dark. Phage titer was determined at
time zero and at intervals of 12 h until the first day, 24 h until the
fifth day, 48 h until the ninth day, 72 h until the twelfth day, 120 h
until the forty-fifth day and 240 h until the end of the experiment
(185 days), by the double-layer agar method. For each phage, three independent
experiments were done.
The survival of V. parahaemolyticus phages was tested in marine
water of the aquaculture system Corte das Freiras, in three different
dates, between May and July 2013. In each date, 50 mL of water were
filtered through 0.45 μm and then by 0.22 μm pore-size membranes
(Poretics, USA) which was followed by the addition of phage suspensions
of about 107 PFU mL−1. The samples were then incubated at
25 °C without shaking, in the dark. Phage titer was determined at
time zero and at intervals of 12 h until the first day, 24 h until the
fifth day, 48 h until the ninth day, 72 h until the twelfth day, 120 h
until the forty-fifth day and 240 h until the end of the experiment
(185 days), by the double-layer agar method. For each phage, three independent
experiments were done.
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