TISSUE PREPARATION. Cubes of peel containing laser-generated pinholes were cut and fixed for 4 h in a 3% gluteraldehyde solution in phosphate buffer of pH 7.2 followed by 3 buffer washes of 10 min.
The peel was then fixed 4 h in a 1% osmium tetroxide solution in buffer followed by 2 buffer washes of 10 min each
Peel was then dehydrated in an acetone series.