Extender
A EK diluent (1.4 g sodium glutamate, 0.14 g potassium citrate×H2O, 0.7 g glucose, 0.2 g D-fructose, 0.7 g inositol, 0.1 g polivinylpyrrolidone, 0.02 g protamine sulfate, 0.98 g anhydrous sodium hydrogen phosphate, 0.21 g anhydrous sodium dihydrogen phosphate were diluted to 100 mL with distilled water; pH 7.3, osmotic pressure 390 mOsmol/kg) [34] was used as the basic extender. Such extender was used throughout the whole experiment and was supplemented with the studied concentrations of antioxidants.
ExtenderA EK diluent (1.4 g sodium glutamate, 0.14 g potassium citrate×H2O, 0.7 g glucose, 0.2 g D-fructose, 0.7 g inositol, 0.1 g polivinylpyrrolidone, 0.02 g protamine sulfate, 0.98 g anhydrous sodium hydrogen phosphate, 0.21 g anhydrous sodium dihydrogen phosphate were diluted to 100 mL with distilled water; pH 7.3, osmotic pressure 390 mOsmol/kg) [34] was used as the basic extender. Such extender was used throughout the whole experiment and was supplemented with the studied concentrations of antioxidants.
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