A simple, sensitive and selective fluorometric method for the determination of nitrite is described. The fluorometric determination is based on the reaction of acetaminophen with nitrite in an acidic medium followed by. alkalinization of the reaction which yields a highly fluorescent product, exhibit l rl s325r430 nm . The ex emoptimum experimental conditions were studied. The fluorescene intensity is linear over a nitrite concentration of. 0.08]1.3mgrml with a detection limit of 2.7 ngrml SrNs3 . The effect of interferences from various metalsrions were studied. The proposed method is accurate, reproducible, shows satisfactory results and compares successfully with the reference method.Q2000 Elsevier Science B.V. All rights reserved.