Chrysanthemum seedlings are treated by sound wave with a certain intensity (100 db) and frequency (1000 Hz) for 3, 6, 9, 12 and 15 days, respectively, and each day for 60 min. The results show that the activity of roots and the content of soluble protein increase greatly under sound stimulation. The activity of plasmalemma H+-ATPase increases while stimulated by sound wave. The concentration of Ca2+, the Ca2+ passage blocker (Verapamil) and the Ca2+ carrier (A23187) can affect the activity of plasmalemma H+-ATPase and the protein kinase inhibitor (Staurosporine) can decrease the activity. The results indicate that the phosphorylation–dephosphorylation process probably regulates the activity of plasmalemma H+-ATPase under sound stimulation.