Autologous fat grafting is commonly used to correct soft-tissue contour deformities. However, results
are impaired by a variable and unpredictable resorption rate. Autologous adipose-derived stromal cells in combination
with lipoinjection (cell-assisted lipotransfer) seem to favor a long-term persistence of fat grafts, thus fostering the
development of devices to be used in the operating room at the point of care, to isolate the stromal vascular fraction
(SVF) and produce SVF-enhanced fat grafts with safe and standardized protocols. Focusing on patients undergoing
breast reconstruction by lipostructure, we analyzed a standard technique, a modification of the Coleman’s procedure,
and three different commercially available devices (Lipokit, Cytori, Fastem), in terms of 1) ability to enrich fat grafts in
stem cells and 2) clinical outcome at 6 and 12 months.