Extract preparation
Extraction was done at room temperature by the simple extraction method. 100 gm coarse powdered plant material was immersed in 200 ml of different solvent (methanol, ethanol and aqueous) contained in 500 ml sterile conical flasks and covered with cotton wool separately. It was placed aside with intermittent shaking for 5 days. They were first filtered with double layered muslin cloth and then through Whatman No. 1 filter paper, and the march was discarded. The filtrate was subjected to evaporation by treating at 40°C in an oven to obtain a dried extract. The dried extract was dissolved in solvent Dimethyl sulphoxide [DMSO] in a ratio of 100mg/ml to determine the antibacterial activity by agar well diffusion assay (AWDA) method.