Methods: The contents of phenols, flavonoids and anthocyanins of the ethanol, ethanol-water
(50/50,v/v) and water extracts of black mullbery were determined using spectrophotometric methods.
Antioxidant assay was based on the measurement of 2,2-diphenyl-1-picrylhydrazyl (DPPH) absorbance
at 517 nm caused by the reaction of DPPH with the test sample, and also on the measurement of 2,2'-
azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) absorbance at 734 nm caused by the reaction
of ABTS with the test sample.