hermostable direct hemolysin produced by Kanagawa
phenomenon-positive strains of V. parahaemolyticus,
a method described previously for the detection of
cholera enterotoxin (4) was applied. Undiluted antihemolysin
serum was mixed with an agar medium at
a final serum concentration of 2.5%. V. parahaemolyticus
strains to be examined were inoculated on the
plate, which was then incubated overnight at 37°C. A
precipitation zone was observed as a halo around
colonies which produced thermostable direct hemolysin.
Test