the morphological parameters were scored from 0 to 3
shown in Table 1 on the basis of criteria defined in study
conducted by (Dwivedi et al., 2012).
Staining quality of four different techniques named
standard Pap staining, MLBC Pap staining, acetic acid
Papanicolaou and MLBC acetic acid pap staining were
assessed by nuclear and cytoplasmic staining quality with
scoring from 0 to 3 given in Table 2. (Alves et al., 2004;
Biswas et al., 2008).
Quality index
After individual evaluation of screening techniques
on the basis of morphological and diagnostic parameter
and subsequent assessment of staining techniques through
nuclear and cytoplasmic staining, overall evaluation of
best techniques among smear preparation and staining was
done by means of quality index originally devised by Chan
et al., 1988. The maximum score possibly attained by one
case, after keeping in view for all of the six morphological
parameters could be 14 and two staining parameters , could
be 4. One the basis of single case the highest attainable
score with one technique was calculated by multiplying the
number of subjects in study with maximum possible score
for each evaluation. A “Quality Index” was then found by
the ratio of obtained score by maximum score: Quality
index=actual score obtained/maximum score possible.