3.4. Serum TEAC
The TEAC values of the samples of blood serum collected from
the rats fed with diets containing CECO and HECO were obtained
from the capacity of the two oils to reduce the ABTS+ radical cation
to ABTS. During this reduction process, the blue–green ABTS+ radical
is decolorised. The extent of inhibition of the absorbance of
ABTS+ was measured with the concentration of Trolox, in order
to evaluate the TEAC. The regression coefficient (r2) calculated
from the calibration curve was 0.9964.
It was observed by plotting the percentage inhibition with time
that 5 min is the appropriate time to measure the absorbance after
mixing ABTS+ with Trolox or phenolic extracts.