No buffering agents were used in enzymatic hydrolysis of whole pretreated slurries. Hydrolysates were sampled at time
points specified in the text, boiled for 10 min and centrifuged at 8000g for 5 min, syringe filtered with a 0.45 lm Minisart (Sartorius, Germany) and stored at -20 ํC for further HPLC analysis.