Methods are based on the
measurement of absorbance at ì
of juice after dilution
with an acidic solvent, but procedures to obtain an
adjusted value of absorbance and to calibrate concentration
of anthocyanins are typical of each method. The
present work is aimed at investigating causes of the
different anthocyanin content resulting from the application
of different methods. Anthocyanins were determined
in a series of blood orange juices according to
the various procedures, including an independent HPLC
analysis (Rapisarda et al., 1994), more expensive and
time-consuming than spectrophotometric methods, but
reliable as a reference point