Resistance to gastric conditions is one of the prerequisite in vitro tests for the evaluation of the probiotic potential of Lactobacillus strains. The in vitro survival test revealed that Lactobacillus isolates (L1, L19, L21, L26 and L40) were most resistant to pH 2 after 3 h of exposure. These results were similar to those of the previous studies, where Lactobacillus strains were viable even after being exposed to pH values of 2.5–4.0, but showed reduced viability at lower pH values (Wang et al. 2010). The variation in acid tolerance of lactic acid bacteria has been linked to the difference in induction of H+-ATPase activity resulting in the removal of protons (H+), alkanization of the external environment, changes in the composition of the cell envelope (Cotter and Hill, 2003).