3- Extraction of bioactive constituents
Weighing of 10gm of Moringa oleifera cake, adding of 100ml of phosphate buffer
(0.1M) with pH 7.5, mix with gentle stirring at 4ºC for 2hours to extract the bioactive
constituents, then centrifuge the contents at 6000 rpm for 30 min, the supernatant was
injected to the Ion Exchange column to separate the bioactive constituents
(Gassenschmidt, U., et al, 1995).