The aliquots were shaken,
added 4 ml of reagent (1 ml of 0.5M FeCl3 in 49 ml of 35% perchloric acid (HClO4)) and vortexed
thoroughly. The samples were incubated at room temperature for 25 minutes and their absorbance was
read at 530 nm. Auxin quantification value was recorded by extrapolating calibration curve made by
using IAA standard (10-100