All changes were found in noncoding sequences and were induced by different
molecular events, such as the insertion of long terminal repeat (LTR) transposon(s), precise miniature
inverted repeat transposable element (MITE) excision, microdeletion, recombination, and a change in the
pool of mitochondrial DNA. For example, the SCAR marker QR is represented by the two variants QR-A
and QR-2. The sequences of the two variants were similar, except for a 457-bp fragment found only in
QR-A; this region was denoted as Q. Region Q was flanked by the direct 3-bp repeat 5-TAA-3 (target site
duplication; TSD) and the inverted 14-bp repeat 5-GGGCCTGTTTGGAA-3 (terminal inverted repeats;
TIRs). These features confer the Q region with similarity to the nonautonomic Tourist-like MITE. In two
groups of independently produced somaclones, the same features (morphological, molecular) were variable,
which confirms the theory of ‘hot spots’ occurring in the genome.