the fish S. dysgalactiae isolates sequences by 5–6 nucleotides.
The phylogenetic tree generated based on the tuf gene sequences
of S. dysgalactiae isolates from fish and other related
Streptococcus species is shown in Fig. 2. Such phylogenetic tree
is obviously revealing that all sequenced fish S. dysgalactiae
isolates belonged to only one cluster, and they were separated
from other related Streptococcus species.
Biased sinusoidal field gel electrophoresis (BSFGE)
All the fish S. dysgalactiae isolates were typeable using
BSFGE. Remarkably, the macrorestriction patterns were
superbly conserved between fish S. dysgalactiae isolates collected
from Japan and Taiwan. Generally, analysis of these
patterns allowed the differentiation of isolates into six types
and one subtype as shown in Fig. 3a. The computer-generated
dendrogram revealed that fingerprint variations obtained by
digestion with SmaI could classify all isolates into three distinct
clusters at a 64% similarity level as shown in Fig. 3b.