Lactobacillus plantarum ACA-DC 146 and L. paracasei subsp. tolerans ACA-DC 4037 were examined for their potential
application as adjuncts in the production of traditional Greek set-type yoghurt. Both strains displayed low milk acidification
activity, while no inhibition was observed towards or from the yoghurt starters used. Yoghurt produced with L. paracasei subsp.
tolerans ACA-DC 4037 exhibited the best sensory properties, with a rich traditional smooth taste, and the strain was selected for
further trials. Yoghurt produced with this strain as an adjunct had good physicochemical properties. After 2 weeks of refrigerated
storage, microbial loads (47.0 log cfu g1) were in accordance with international recommendations and guidelines for probiotic and
starter cultures in milk products. Increasing the microbial load further, using concentrated and encapsulated inocula
(10–11 log cfu g1), gave yoghurt with long fermentation times and poor organoleptic properties