granulesAn overview of the intrinsic characteristics of the native AOand control starches was established by analyzing their molarmass, crystallinity and melting behavior. For molecular size andmolar mass distributions, the starches were dissolved and ana-lyzed by asymmetrical flow field-flow fractionation coupled withmulti-angle laser light scattering (A4F-MALLS). The solubilizationrecoveries were higher than 92% for all samples and the elutionrecoveries were all higher than 90%, providing representative datafor the samples. The size distribution of the native control starchexhibited two populations corresponding to amylose (RH6–35 nm)and amylopectin (RH30–390 nm, Fig. 1) (Rolland-Sabate et al.,2011). The¯
Mwof AO being 9.1 × 106g mol−1was about 24-timeslower than the control and exhibited a lower¯
RG(199 nm) anda lower dispersity (¯
Mw/¯Mn= 26, Table 1), as is expected for anAO starch. However, these values were high compared to typi-cal pure amylose samples, but were in line with the presence oflarge particles, which could be residual branched macromoleculesor aggregates (Rolland-Sabate et al., 2011).