This issue suggested that a careful optimization of the procedure
was needed prior to applying it to honey. The optimization work
mainly addressed the program of the chromatographic elution and
a proper choice of the UV wavelength in order to maximize both
resolution and sensitivity. An aqueous solution of trifluoroacetic
acid (0.025%, v/v), solution A, and acetonitrile, solution B, were still
adopted as components of the mobile phase, whereas the optimized
elution program for honey matrix is reported in Table 1.